The chicken HPRT gene: a counter selectable marker for the DT40 cell line

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The chicken HPRT gene: a counter selectable marker for the DT40 cell line.

We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will ...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1999

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/27.9.1966